TAPe5 Tag - Optimized technology for the purification of proteins and protein complexes
Referenznummer TO 01-00679
Challenge
Recombinant proteins play an important role not only as a research tool but also in industrial applications. For both fields of application a high purity as well as a high yield of the isolated protein are essential. Affinity tags attached to the protein of interest are commonly used in purification procedures combining easy handling and efficient purifica-tion.
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Fig. 1: Tandem affinity purification of a kinase protein complex from HEK293 cells by using the TAPe5-tagged kinase domain as a bait, colloidal Coomassie staining.
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Technology
The novel TAPe5 Tag technology provides a highly efficient tandem affinity system for recombinant protein purification and detection. It offers the following advantages:
- short tag of less than 5 kDa
- rapid and gentle affinity purification under physiological conditions
- elution of bioactive proteins and intact protein complexes
- purification to homogeneity
- no protease-step required
This system is an extremely fast and efficient method for the purification of recombinant proteins via a StrepII- as well as a Flag-Tag. As the purification scheme does not include proteolytic steps this new method is more convenient and feasible by main-taining its excellent purification qualities.
Commercial Opportunity
In-licensing opportunity
Patent Situation
An US patent application was filed in May 2006.
Further Reading
den Hollander et al. (2007) Nat. Genet., Vol 39, p. 889
Gloeckner et al. (2007) Proteomics, Vol. 7, p. 4228
