The Cd19 promoter specifically directs expression at the earliest stages and throughout B-lymphocyte development and differentiation. A Cre cassette is inserted into the Cd19 exon 2, functionally disrupting the gene. Homozygous mice are Cd19-deficient, whereas heterozygous mice are phenotypically normal and can be used for specific deletion of floxed targets in B-lymphocytes. Mice that are homozygous deficient for Cd19 are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. A deficiency in the B-1 subset of B-lymphocytes is observed along with a concomitant reduction in serum IgM. Homozygous mice are severely impaired in their ability to respond to T-cell-dependent antigens and fail to form splenic germinal centers.
Developmental Biology Research (Cre-lox system) - cre related,
Cre-lox-System, Genetics Research (Mutagenesis and Transgenesis: Cre-lox system) - Cd19tm1(cre)Cgn related
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency (B cell defects)
Further Reading & Data Sheet
This animal model is bred and managed by The Jackson Laboratory and marketed for commercial use by Ascenion.