Short Term Isotope Pulse Labeling Method for Analyzing Metabolic States and Drug Targets in Biological Samples
Keywords
pSIRM, metabolomics, metabolic dynamics, cellular metabolism, spectrometry, drug development
Invention Novelty
Here we present pulsed stable isotope resolved metabolomics (pSIRM) as a tool for dynamic metabolic characterization of cellular metabolism.
Value Proposition
pSIRM will contribute to unravelling metabolic dynamics in complex metabolic networks, to identify drug targets and, most importantly, to measure drug action regarding its dynamics, on-target and off-target effects. pSIRM harbors the potential to make drug development much more efficient and cost effective.
Scheme of a possible pSIRM experiment to measure the direct response after a drug treatment and later adaptive changes of metabolism (S. Kempa, Max-Delbrück-Center).
Technology Description
We have adapted spectrometric methods for metabolomic profiling and stable isotope resolved metabolomics. In addition, we improved robustness and reproducibility of results and implemented a strategy for absolute quantification of metabolites. The technology was designed to enable automated processing of cell cultures during experimental procedures. Starting from 1.000.000 cells, we are now able to measure stable isotope profiles from as little as 500 cells – thereby saving on ingredients as buffers or stable isotopes and enabling simultaneous handling of diverse sets of experiments.
Commercial Opportunity
We are looking for industrial partners to cooperate on further optimizing, automating, and evaluating pSIRM technology. Licensing is also possible.
Development Status
Proof of concept demonstrated, mechanistic studies of immune and cancer cells, characterization of metabolic drugs, identification and characterization of severe off-target effects.
Patent Situation
Patents granted in EP (EP2944963B1) and US (US10082499B2); priority date: May 14, 2014.
Further Reading
doi:10.1002/ijc.33423
doi:10.1161/CIRCULATIONAHA.120.052788
doi:10.1016/B978-0-12-801329-8.00009-X
doi:10.1038/nature22353
doi:10.1080/14756366.2021.1935917
doi:10.1186/2049-3002-2-9
Institute of Origin
