CrispRGold - Design of efficient CrispR/Cas sgRNA sequences and off-target prediction
Keywords
CRISPR/CAS, gene editing, gene therapy, sgRNA, off-target prediction
Invention Novelty
MDC scientist developed a web-based software tool to design highly specific sgRNA sequences for CRISPR/Cas-based gene editing.
Value Proposition
The design of sgRNA sequences that specifically match the sequence of the target gene is a crucial step and efficiency of CRISPR/Cas genome editing directly depends on sgRNA sequence. CrispRGold, written by MDC scientists, helps to identify the most effective and specific RNA sequences. Furthermore, the tool provides an excellent off-target-prediction feature.
Commercial Opportunity
Licensing.
Technology Description
CrispRGold is a sgRNA design tool that searches for optimal sgRNA candidates in given genes or sequences. CrispRGold goes through iterative loops with decreasing stringency to find sgRNAs. The most important criterion for the design is the specificity, which is calculated based on genome-wide alignments of each candidate sgRNA and subsequent analysis of the mutation distance of each potential offtarget-site. In addition to the specificity, CrispRGold filters for parameters important for the efficiency. The algorithm is based on experimental data, especially regarding the uniqueness of sequences.
Commercial Opportunity
Licensing.
Development Status
Market.
Further Reading
Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line. Chu et al., PNAS 2016. PMID: 27729526
Licensing Contact
Dr Céline Christiansen-Mensch
Technology ManagerT: +49 (0)1590 4836722
christiansen@ascenion.de
Institute of Origin
