Injection tunnel for stress-reduced handling of laboratory mice

Reference Number TO 32-00174

Keywords

Injection tunnel, intraperitoneal injection, laboratory mice, stress reduction, animal research, refinement methods, 3D printing, 3R, animal welfare

Invention Novelty

The present invention describes a specially developed injection tunnel that greatly simplifies the administration of injections (both intraperitoneally as well as intravenously) as well as tail vein blood sampling in laboratory mice while reducing stress for the animals. Made of synthetic resin and lined with fur, it provides a dark, soft, and comfortable environment that mice enter voluntarily (Fig. 1). Its narrow dimensions prevent turning, allow natural positioning, and ensure safety for both animals and handlers. The minimal physical contact during injection lowers stress, confirmed by reduced corticosterone in fur and increased immunoglobulin A in saliva. The design therefore offers a novel, practical, and welfare-oriented alternative to existing techniques, enhancing both research efficiency and animal well-being.

Value Proposition

The injection tunnel improves intraperitoneal injections in laboratory mice by making the process safer, more efficient, and animal-friendly. Complying with EU Directive 2010/63/EU and the German Animal Welfare Act, it reduces stress and suffering while lowering the risk of bite injuries. Its simple design enables safe use by less experienced staff, shortening training times and lowering anxiety. It is easily integrated into existing workflows and meets the growing demand for refinement technologies, offering significant market potential in Germany and abroad.

Fig 1. Prototype of the injection tunnel

Technology Description

The injection tunnel is designed for stress-reduced intraperitoneal injections in laboratory mice. An integrated base stops rolling, and 3D printing allows adaptation to different sizes. Removable caps ensure that mice remain in the tunnel as long as needed while allowing easy exit to the front once the caps are removed. Autoclavable and alcohol-resistant, it ensures easy cleaning and reuse. Scientific studies confirm significant stress reduction, highlighting its suitability for widespread adoption in research facilities focused on animal welfare and procedural efficiency.