Mice homozygous for the Igk-Ctm1Cgn targeted mutation are viable and fertile. The number of B cells expressing l chain is increased 2-fold in heterozygotes and 7-fold in homozygotes. Immunology and Inflammation Research: CD Antigens, Antigen Receptors, and Histocompatibility Markers, Immunodeficiency
Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. Homozygous mice lack B220+ peripheral blood lymphocytes. This mutant mouse strain represents a model that may be useful in studies related to B-cell immunodeficiency Immunology and Inflammation Research:Immunodeficiency (B cell deficiency) Research Tools:…
Mice homozygous for the Igh-5tm1Cgn targeted mutation are viable and fertile. Homozygous mutant mice show a delay in affinity maturation although they are able to generate high affinity B cell memory. Heterozygous mice show a disproportionate percentage of B cells expressing the wildtype IgH allele. Also known as IgD. Immunology and Inflammation…
Mice homozygous for the Igl-5tm1Cgn mutation are viable and fertile. Homozygotes show a block in B cell development in bone marrow at the pre-B cell stage; however, blockage is leaky and a low percentage of B cells do get to the peripheral immune system. Immunology and Inflammation Research: CD Antigens, Antigen Receptors, and Histocompatibility…
Mice homozygous for the Igh-6tm1Cgn targeted mutation are viable and fertile. Homozygous mutant mice lack mature B-cells. There is no expression of membrane-bound IgM, although some B-cells may be produced using a C gene other than mu. It may be useful as a model for B-cell immunodeficiency found in humans. Also know as muMT. Immunology and…
Mice homozygous for the Igh-Jtm1Cgn targeted mutation fail to produce functional B-cells as they lack the gene for the heavy chain joining region. Also known as JHT. Immunology and Inflammation Research: Immunodeficiency (B cell deficiency) Research Tools: Immunology and Inflammation Research (B cell deficiency)
Mice homozygous for the Igh-6tm1Cgn targeted mutation are viable and fertile. Homozygous mutant mice lack mature B-cells. There is no expression of membrane-bound IgM, although some B-cells may be produced using a C gene other than mu. It may be useful as a model for B-cell immunodeficiency found in humans. Also know as muMT Immunology and…
Mice homozygous for the Igh-5tm1Cgn targeted mutation are viable and fertile. Homozygous mutant mice show a delay in affinity maturation although they are able to generate high affinity B cell memory. Heterozygous mice show a disproportionate percentage of B cells expressing the wildtype IgH allele. Also known as IgD. Immunology and Inflammation…
Mice homozygous for the Cd5tm1Cgn targeted mutation are viable and apparently healthy. Both T and B cells in homozygous mutant mice lack surface CD5. Mice show normal immune responses to both T cell-dependent and -independent antigens. Immunology and Inflammation Research: CD Antigens, Antigen Receptors, and Histocompatibility Markers
These transgenic mice carry the Cre recombinase gene under the control of the rat nestin promoter. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing Cre, generating a proportion of mosaic animals. All adult organs, including germ-line cells, may be affected. The balancer 2 line…
These transgenic mice carry the Cre recombinase gene under the control of the rat nestin promoter. When crossed with a strain carrying a gene flanked by loxP sites, partial deletion of the loxP-flanked allele occurs before embryonic day 10.5 and is detectable in all adult organs examined including germ line cells. Because of the partial deletion,…
The Cre recombinase is under the control of the Mx1 promoter. This promoter is silent in healthy mice, but can be induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA. When combined with a mutant carrying a gene that has been flanked by loxP recognition sites, the…
In this transgenic strain, deletion of loxP-flanked genes occurs in all tissues, including germ cells. The Cre gene in this strain is under the transcriptional control of a human cytomegalovirus minimal promoter and is likely to be expressed before implantation during early embryogenesis. It also appears to be X-linked since transgene transmission…
The Cre recombinase is under the control of the Mx1 promoter. This promoter is silent in healthy mice, but can be induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA. When combined with a mutant carrying a gene that has been flanked by loxP recognition sites, the…
Cyclic di-Nucleotides as adjuvants (TO 02-00158)
02-00158
Intra-nasal (mucosal) vaccination.
This invention introduces a groundbreaking approach to mucosal vaccine delivery by utilizing cyclic di-nucleotides, such as c-di-GMP and c-di-AMP, as potent adjuvants. These naturally occurring bacterial signaling molecules mimic the early stages of…
Prokaryotic expression systems are widely used for the production of recombinant proteins, but often encounter problems to obtain sufficient yields of the functionally active gene products. Most challenging is the formation of inclusion bodies, incorrect folding of the expressed protein, protein toxicity for the producer cells and protein…
Challenge Genetically modified animals are essential research tools in modern neuroscience, since they allow researchers to study the role of specific genes in pathological processes leading to neurodegenerative diseases. The myelin-associated glycoprotein (MAG) is a transmembrane glycoprotein localized in the myelin sheath membranes of periaxonal…
Challenge Genetically modified animals are essential research tools in modern neuroscience, since they allow researchers to study the role of specific genes in pathological processes leading to neurodegenerative diseases. Tenascin-R (TNR) is an extracellular matrix glycoprotein expressed predominantly in the central nervous system and localizes to…
Challenge Genetically modified animals are essential research tools in modern neuroscience, since they allow researchers to study the role of specific genes in pathological processes leading to neurodegenerative diseases. The close homologue of L1 (CHL1), a member of the L1 family of cell adhesion molecules, is abundantly expressed by neurons and…
