Challenge Poly(ADP-ribose) polymerase-1 (PARP-1) catalyzes the modification of nuclear enzymes by (ADP-ribosyl)ation. PARP-1 function was first associated with DNA single-stranded break repair, especially in tumor cells, and maintenance of genomic integrity. Accumulating evidence suggests that PARP-1 also plays important roles in other disease…
Challenge The zebrafish is a relevant model system for studying vertebrate development and organogenesis due to its short life cycle and the easiness of isolating large numbers of mutants. For the simplicity of its pronephros it suits especially for the analysis of kidney development. The Wilms tumour protein WT1 is an essential factor for kidney…
Homozygous mice are IL10-deficient and develop spontaneous colitic lesions in the cecum and large intestine as early as 6-8 weeks of age. The severity and age of onset of colitis varies with the donor type and location of the cytokine deficiency colitis susceptibility 1 (Cdcs1) interval. Generally, the C57BL/6 region encompassed byD3Mit348 through…
Mice homozygous for the Il10tm1Cgn targeted mutation are viable and fertile when housed under SPF conditions. Under conventional housing conditions homozygous mutant mice are small in size, anemic, and have chronic enterocolitis. It should be noted that the phenotype of cecocolitis in this strain requires an interaction with an as yet undefined…
Mice homozygous for the Il2rgtm1Cgn targeted mutation are viable and fertile. Mutant mice have hypoplastic thymuses with a 10-fold reduction in the absolute number of lymphocytes. They have a limited number of mature splenic B and T cells, lack NK cells and Peyer's patches, and development of gut-associated intraepithelial lymphocytes is…
Mice homozygous for the Il10tm1Cgn targeted mutation are viable and fertile when housed under SPF conditions. This mutant develops type 1 diabetes at the same rate as the NOD/Lt parental strain. The Il10 tm1Cgn mutation also renders this NOD/Lt stock susceptible to colitis (although not as severe as other strains of Il10 deficient mice) when…
Mice homozygous for the Il10 and Il4 targeted mutations are viable and fertile when housed under SPF conditions. NOD/Lt mice deficient for both of these genes develop type 1 diabetes at a rate equivalent to the parental strain. The Il10 tm1Cgn mutation also renders this NOD/Lt stock susceptible to colitis (although not as severe as other strains…
Mice homozygous for the Il10tm1Cgn targeted mutation are viable and fertile when housed under SPF conditions. Under conventional housing conditions homozygous mutant mice are small in size, anemic, and have chronic enterocolitis. It should be noted that the phenotype of cecocolitis in this strain requires an interaction with an as yet undefined…
Mice homozygous for the Il10tm1Cgn targeted mutation are viable and fertile when housed under specific pathogen-free (SPF) conditions. The phenotype of Il10-deficient mice is strongly influenced by the genetic background. At 3 months of age, Il10tm1Cgn homozygous mutant mice on a BALB/c background exhibit severe intestinal lesions, increased…
Mice homozygous for the Ncam1tm1Cgn targeted mutation show a 10% reduction in overall brain weight and 36% reduction in olfactory bulb size. Motor end plates were 14% smaller in NCAM-deficient mice compared to controls, and the formation of junctional folds was delayed. They also show deficits in spatial learning and in the amount of protein-bound…
Mice homozygous for the Il4tm1Cgn targeted mutation are viable and fertile. T and B cell development is normal but IgGl and IgE levels and the ability of homozygous mutant mice to produce Th2-derived cytokines are significantly reduced. Cancer Research: Growth Factors/Receptors/Cytokines Immunology and Inflammation Research: Growth…
Mice homozygous for the Il10tm1Cgn targeted mutation are viable and fertile when housed under SPF conditions. Under conventional housing conditions homozygous mutant mice are small in size, anemic, and have chronic enterocolitis. Intestinal problems are far less severe (local inflammation of the proximal colon) in a pathogen-free environment.…
Mice homozygous for the Il10tm1Cgn targeted mutation are viable and fertile when housed under SPF conditions. Under conventional housing conditions homozygous mutant mice are small in size, anemic, and have chronic enterocolitis. Intestinal problems are far less severe (local inflammation of the proximal colon) in a pathogen-free environment.…
Mice homozygous for the targeted mutation are viable and fertile when housed under SPF conditions. At 3 months of age mutant mice exhibit severe intestinal lesions, increased granulocyte counts and elevated serum amyloid A levels. There is a 67% prevalence of colorectal adenocarcinoma. This mutant mouse strain represents a model that may be useful…
These mice are homozygous for four different induced mutations. The cumulative result of these mutations is a mouse model in which hypercholesterolemia can be reversed. By themselves, the combined presence of the Ldlrtm1Sgy and Apobtm1Sgy targeted alleles results in mice with a high susceptibility to atherosclerosis and total plasma cholesterol…
This strain was derived from a cross between mice of strains NOD.B6-Itgb7tm1Cgn/Lt (Stock No. 004310), then at backcross generation N9, and NOD.129P2(B6)-Selltm1Tft/1Lt (Stock No. 3860), at backcross generation N10. Dr. Leiter transferred this stock to the T1DR in 2005. Selltm1Tft related Immunology and Inflammation Research: CD Antigens,…
This strain was derived from an initial cross between mice of strains NOD/Lt and C57BL/6-Itgb7tm1Cgn (Stock No. 002965), followed by 10 backcrosses (to N11) to NOD/Lt of progeny selected for heterozygosity for the targeted allele. Heterozygous offspring of the final backcross were intercrossed to initiate a line homozygous for Itgb7tm1Cgn, and the…
Mice homozygous for the Itgb7tm1Cgn targeted mutation have a marked hypoplasia of the gut-associated lymphoid tissue (GALT). This deficit is apparently due to the failure of Itgb7-deficient lymphocytes to arrest and adhere in the GALT. Cell Biology Research: Defects in Cell Adhesion Molecules Immunology and Inflammation Research: Growth…
Transgenic mice carrying the Igh-6tm1Cgn allele are viable, fertile, and normal in size and restores the B220+ B-cells. The number of B-cells is reduced and the ratio of CD4 to CD8 is normal. Although only a fraction of the transgenic mice have an increased amount of insulitis, it is significantly more severe, especially in males. No diabetes was…
Transgenic mice carrying the Igh-6tm1Cgn allele are viable, fertile, and normal in size and restores the B220+ B-cells to normal. The number of B-cells is normal and the ratio of CD4 to CD8 is normal. Although only a fraction of the transgenic mice have an increased amount of insulitis, it is more severe, especially in males. Diabetes incidence is…
