CD3-CAR NK-92 cell-mediated TCR+ depletion (TO 32-00124)
32-00124
Fig. 1 A: Manufacturing of allogeneic “off-the-shelf” CAR T cells by means of TCR/CD3-specific CAR NK-92 cell lines for the removal of residual TCR+ AT-cells from TCR-deleted CAR T-cell product. B: CAR NK-92 cells allowed to produce TCR– CAR T cells with <0.01% TCR+ T…
Nutritional compositions for treating dysfunction of liver fat metabolism – VAFD (TO 11-00065)
11-00065
Vitamin-A Fasting Diet (VAFD) rescues impairments in liver fat metabolism, thereby reducing the risk of cirrhosis, liver cancer, hyperlipidaemia, cardio-vascular disease, and muscle loss (sarcopenia), @Rudolph
Here we provide the…
Novel Lectin-Targeting therapeutics and diagnostics for Pseudomonas aeruginosa infections
(TO 02-00396)
02-00396
Diagnostic approach using the antibiotic prodrug (modified from Zahorska et al. and Meiers et al.)
Three distinct approaches targeting bacterial lectins LecA and LecB, critical components in the biofilm formation of…
Prediction for targeting efficiency of guide RNA for a gene of interest in bacteria (TO 02-00380)
02-00380
Workflow of prediction of guide efficiency [Yu et al. 2022, modified]
Provided is a machine learning-based tool that improves reliable prediction of targeting efficiency of guide RNA for gene silencing in bacteria.
CRISPR interference…
Human TCR constructs for adoptive cell therapy of cancer (TO 03-00553)
03-00553
Mutant Rac1/2-specific TCR-transduced T-cells elicited tumor regression upon adoptive T-cell therapy (ATT) in a mouse model of Rac1P29S-positive tumors (Immisch et al., Front Immunol. 2023;14:1119498)
The invention provides novel high-affinity T-cell receptors…
Activity-enhanced variant of hyperactive Sleeping Beauty transposase SB100X (TO 03-00542)
03-00542
In the last decade, gene therapies have experienced a breakthrough, with most strategies initially focusing on viral vector systems, even though these are quite difficult and expensive to manufacture on a larger scale. Accordingly, given their…
Safer Sleeping Beauty transposase variants with improved integration profiles (TO 03-00541)
03-00541
In the last decade, gene therapies have experienced a breakthrough, with most strategies initially focusing on viral vector systems, even though these are quite difficult and expensive to manufacture on a larger scale. Accordingly,…
SCON – Artificial intron devoid of hypomorphic target gene effects for one-step generation of conditional animal models (TO 43-00017)
43-00017
a) Scheme of SCON integration into the eGFP sequence and removal by Cre-recombinase; b) eGFP expression analysis [Source: Scheme provided by IMBA]
“SCONs” are artificial “Short COnditional INtrons”…
Broadly neutralizing antibodies against hepatitis E virus (HEV) (TO 18-00052)
18-00052
pORF2 glycosylation site N562 discriminating between infectious and non-infectious HEV particles
Scientists of TWINCORE – Centre for Experimental and Clinical Infection Research and Lübeck University identified a novel set of human antibodies specifically…
Advancing Bioproduction: HDLBP overexpression enhances protein production and secretion (TO 03-00515)
03-00515
Fig 1 A) Relative expression of EPO-Fc in the cell culture medium from cells without HDLBP overexpression (parental cells) and HDLBP-overexpressing CHO cells (HDLBP+ cells) stably transfected with EPO-Fc (EPO WT) and codon-optimized…
Th1 cells play a central role in the immune system and mediate protective as well as pathologic functions. Activated Th1 cells are characterized by IFN-gamma production which via a feedback-loop promotes differentiation of naïve CD4+ cells into Th1 cells. Th1 cells act on the cellular immune system by maximizing the killing efficacy of macrophages…
Elevated TNF levels are a hallmark of inflammation. Therefore, agents blocking TNF have offered patients tremendous improvements in the treatment of various autoimmune diseases including rheumatic arthritis, systemic lupus erythematosus or Chron's disease. Biologics like the antibody adalimumab (Humira®, sales 2015: 14 Bn USD) are still hugely…
BreakTag - Novel method for detection of ds-breaks and analysis of CRISPR-induced on- and off-target effects (TO 40-00003)
40-00003
Schematic illustration of the BreakTag workflow [provided by IMB; click to enlarge]
BreakTag is a high throughput method for detecting the locations and end-structure of on- and off-target DNA double-strand…
Reprogramming immune cells for CAR cell-based therapies (TO 32-00082)
32-00082
Novel CD3-zeta gene editing strategy enables redirection of T cells and NK cells with chimeric antigen receptors (D. Wagner)
The invention comprises an optimized CRISPR-Cas9-mediated gene transfer for efficient integration of novel truncated chimeric antigen…
Fiber Membrane Oxygenator with Minimized Priming Volume (TO 32-00108)
32-00108
Priming volume minimization by microspheres in the blood compartment (a) and certain fiber configuration of the gas compartment (b) of the novel fiber membrane oxygenator. (source: PCT/EP2022/080548, modified)
Researchers at Charité - Universitätsmedizin…
Particle-mediated transport of dissolved active agents into hair follicles (TO 32-00091)
32-00091
Fig. 1: Confocal microscopic image of a hair follicle. Source: Charité
The delivery of particles into the hair follicle is known, however, there has been no efficient means of delivering dissolved active ingredients into hair follicles, such as…
Non-integrating alpharetrovirus-based vector particles for efficient RNA delivery and targeted gene modification
(TO 15-00728)
15-00728
Non-integrating alpharetrovirus-based viral-like particles for efficient non-viral RNA delivery into target cells. As an application example, a.Gag.MS2CP VLPs can simultaneously deliver CRISPR/Cas9 RNA…
Liver stem cell generation for therapeutic applications (TO 15-00446)
15-00446
Liver stem cell generation and autologous gene therapy
Researchers of Hannover Medical School (MHH) have developed a novel process for generating liver stem cells capable to differentiate into various liver cell types (hepatocytes, cholangiocytes, liver sinusoidal…
Novel Treatment of Pulmonary Arterial Hypertension (PAH) (TO 15-00651)
15-00651
LC–MS analysis revealed significantly higher levels of PGE2 in HUCMSCs. (Hansmann et al., 2022)
The invention comprises a novel therapeutic approach for the treatment of Pulmonary Arterial Hypertension (PAH) based on cell culture supernatant derived from…
